Distrust 和 mistrust 當 (及物) 動詞用時均意為「不信任，不相信，懷疑」，通常可以互換，如 Somehow I distrust/mistrust the whole business. (不知怎麼地，我就是不相信這整件事) ；He distrusts/mistrusts banks, so he keeps his money at home. (他認為銀行靠不住，所以把錢存在家裡)。
然而，distrust 比 mistrust 要來得常用，且含有「猜忌；猜疑」(suspicion) 的意思，所以當某人曾經有欺騙或其他負面行為或某事物曾經造成危害或產生不良影響，或者當事人認為某人或某事物可能會做出上述行為或發生上述情況，因而對該等人事物產生不信任感或懷疑時，一般都使用 distrust 而不用 mistrust，如 I have known him to be deceitful in the past and I have come to distrust everything he says. (我知道他過去愛騙人，所以我對他所說的事情一概不信)；Most people distrust politicians. (大多數人都不信任政客)；He distrusts her because he thinks she’ll cheat him. (他不信任她，因為他認為她會騙他)。
Mistrust 是指比較不確定或不是很肯定的「不信任」或指對自己相關事物的「懷疑」，如 There was something about her manner that made me uneasy and I found myself beginning to mistrust her. (某件跟她態度有關的事情令我不安，結果我發現自己開始不信任她)；She told herself she had no reason to mistrust him. (她告訴自己沒有理由不信任他)；Why do you mistrust him so much? He seems honest enough to me. (你為何如此不信任他呢? 我覺得他似乎很老實)；I mistrust my own judgment when it comes to my appointment of Mike as new English teacher. (談到我聘任麥可為新的英文老師一事，我懷疑自己的判斷力)。
Distrust 和 mistrust 亦皆可當 (不可數) 名詞用，意思也同樣為「不信任，不相信，懷疑」，其後經常與介系詞 of 連用，如 The child looked at the stranger with distrust. (那孩子用懷疑的目光望著陌生人)；I have a distrust of airplanes. (我對飛機信不過)；Mistrust between the two countries is intense. (兩國間彼此非常不信任)；He has a deep/strong mistrust of anything new. (他對任何新事物都極不信任)。
再者，distrust 和 mistrust 的形容詞分別為 distrustful 和 mistrustful，兩者均意為「不信任的，不相信的，懷疑的」，其後亦經常與介系詞 of 連用，但 mistrustful 有時也與 toward 連用，如 The old woman is distrustful of her children’s intentions. (這位老婦人懷疑她子女的意圖)；They were profoundly distrustful of anything new. (他們對任何新事物都極不信任)；She was mistrustful of Frank’s loyalty. (她懷疑法蘭克的忠誠)；My wife was becoming increasingly mistrustful toward doctors. (我太太越來越不相信醫生)。